Scalable, high performance, enzymatic cathodes based on nanoimprint lithography

• Dmitry Pankratov,
• Richard Sundberg,
• Javier Sotres,
• Dmitry B. Suyatin,
• Ivan Maximov,
• Sergey Shleev and
• Lars Montelius

Beilstein J. Nanotechnol. 2015, 6, 1377–1384, doi:10.3762/bjnano.6.142

• of our knowledge, this is one of the first reports concerning the usage of nanoimprint lithography for amperometric bioelectronic devices. The enzyme (Myrothecium verrucaria bilirubin oxidase) was immobilised on planar (control) and artificially nanostructured, gold electrodes by direct physical

• : bilirubin oxidase; bio-electrocatalysis; direct electron transfer; nanoimprint lithography; oxygen reduction reaction; Introduction Reduction of oxygen (O2) is the key reaction in many natural and artificial systems, and indeed, this reaction is one of the most interesting research issues in both academia

• commercially available MCO, bilirubin oxidase (BOx), which is one of the main biocatalysts exploited today to design third-generation (i.e., direct electron-transfer-based), O2 reducing biodevices (e.g., O2-sensitive biosensors [18] and biocathodes of enzymatic fuel cells [19]). Contrary to many other MCOs